Coding

Part:BBa_K1966003:Experience

Designed by: Brandon Wilbanks   Group: iGEM16_UT-Knoxville   (2016-10-11)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1966003

This part has been shown to be functional for the following activities:

1)conversion of toluene to benzaldehyde
2)conversion of m-xylene to m-tolualdehyde
3)conversion of p-xylene to p-tolualdehyde

The figure below demonstrates conversion of toluene, m-xylene, and p-xylene to the respective aldehydes as described above. This part was not successful in conversion of 3-chlorotoluene, m-anisole, and p-anisole, which is also seen below.

Fig8.1.jpg

The 3 GC-MS chromatograms below show the results of conversion of each substrate after 8 hours of incubation with E. coli BL21 DE3 containing part BBa_K1966003.

Mxylenechromo.png Pxylenechromo.png Toluenechromo.png

Further characterization will be attempted for the following functions:
1)conversion of 3-chlorotoluene to 3-chlorotolualdehyde
2)conversion of m-anisole to m-anisaldehyde
3)conversion of p-anisole to p-anisaldehyde

Registry information will be updated as to whether or not these functions have been shown.

User Reviews

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